Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I. Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier.
Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract. Each one of the nine tissue field samples suions pigs eem as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed.
Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments. This was possible due to the high annealing temperature of the primers pair designed and contributes to the reaction efficiency. Seven tissue samples from clinically healthy animals were negative for PCR amplification data not shown. This assay was based on the amplification of a highly conserved viral gD gene fragment.
The PCR for PRV genome detection is also an important method in screening pig specimens collected for xenotransplantation to increase the safety of organ transplantation 7 and to detect viral infection in a wide spectrum of species reported to be susceptible to PRV, through either natural or experimental infections 8.
Doença de Aujeszky
Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4. The annealing temperature and number of cycles were determined experimentally. Also, the BLAST search against nucleotide databases of different herpesvirus and random doens sequences revealed this region is very specific for PRV genomes.
Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR.
Published online Sep 1. Replication in the respiratory tract, central nervous system and reproductive organs is responsible for pathological changes causing different disorders Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR.
World Organization for Animal Health. A rapid and accurate diagnosis of PRV infection is important for the initiation of appropriate control strategies. The development, optimization and aujesziy of a polymerase chain reaction PCR assay are presented for the aujeszzky of pseudorabies infection.
The analytical sensitivity of the test was estimated to be 1.
Moreover, the viral genomes of a related herpesvirus and other DNA genome porcine viruses as follow: The PCR assay described here provides a rapid, highly sensitive, and cost-effective laboratory diagnosis for pseudorabies infections. PRV specific primers were designed using the Oligo 6.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
However, this method ajjeszky time-consuming and false negative results may occur in submissions from latently infected animals This region was highly conserved for all reported genomes as shown by aligning of these sequences. The trigeminal ganglion is the most consistent site for virus isolation, although latent virus is usually difficult to culture or even impossible 113 and PCR is the method recommended to detect viral genome present in this site.
The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. The analysis directly from clinical samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. This paper describes the development, optimization and performance assessment of a rapid and ajueszky sensitive PCR test for detection of pseudorabies virus.
National Center for Biotechnology InformationU. All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License.
Author information Article notes Copyright and License information Disclaimer. Journal List Braz J Microbiol v. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis. Iowa State University Press; Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6.
The analytical sensitivity of the test was consistently observed to be 1.
Specificity of the PRV amplicons was furthermore confirmed by Sma I restriction endonuclease analysis which generated the two expected fragments of and bp in length Fig. The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs but it is the recommended test for detect PRV latent infection.
Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and pigs with emphasis on respiratory tract. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs.